Efficacy | Evaluation Model | Evaluation Indicator |
Anti-wrinkle | Elastase inhibition experiment | Elastase activity inhibition rate |
Collagen inhibition experiment | Inhibition rate of collagenase | |
UVA-induced model of primary fibroblast aging | Measurement of ROS content, staining of β-galactosidase, and mitochondrial membrane potential | |
UVA-induced model of primary fibroblast aging | Type I collagen and elastin content | |
UVA-induced model of primary fibroblast aging | Expression of elastin degradation related genes (MMPs) | |
UVA-induced model of primary fibroblast aging | Expression of aging-related genes (P53, P21, P16) | |
Firmness | Elastase inhibition experiment | Elastase activity inhibition rate |
Primary fibroblast aging model | Type I collagen and elastin content | |
Cell-aging Model | UVB-induced photoaging model in epidermal stem cells | Measurement of ROS content, staining ofβ-galactosidase, mitochondrial membrane potential and stemness (integrinα6 and integrin β1) expression |
UVA-induced model of fibroblast aging | Measurement of ROS content and expression of aging-related genes (P53, P21, P16) | |
Advanced glycation end products (AGEs) induced model of primary fibroblast aging | Measurement of ROS content and expression of aging - related genes (P53, P21, P16) | |
Anti-oxidant | DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay | DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay free radical scavenging rate |
PTIO (2-phenyl- 4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide) radical scavenging assay | PTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide) free radical scavenging rate | |
Induction of oxidation stress model in HaCaT by UVB | Free radicals including ROS, SOD | |
Anti-glycosylation | Bovine serum albumin (BSA) - Fructose Maillard reaction simulation | Inhibition rate of advanced glycation end products (AGEs) |
High glucose-induced fibroblast glycation model | AGEs (CML), measurement of RAGE content, and measurement of glucose uptake | |
Glycation model of natural aging in primary fibroblasts | AGEs (CML), measurement of RAGE content |